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For CTB+NeuN+ quantification after injections in the CC, 250 NeuN+ cells (50 per layer) were randomly selected within a region of interest (ROI) depicting a column in the S1-Bf on the hemisphere contralateral to the injection site
from the top result below
https://www.science.org/doi/10.1126/sciadv.adw5487
Early fate diversification of radial glial progenitors during corticogenesis | Science Advances