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This bias can distort apparent protein distributions, as evidenced by PolII mapping where sonicated chromatin showed a broad peak centered at the transcription start site, while MNase-based mapping accurately aligned with the polymerase's active site at +35 bp [49]

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Bioinformatics Research Hub

https://www.bioinforesearch.com/posts/histone-chip-crosslinking-methods-compared-from-formaldehyde-to-dual-fixation-for-epigenetic-research

Bioinformatics Research Hub Home Research About Contact Home Research About Contact Histone ChIP Crosslinking Methods Compared: From Formaldehyde to Dual Fixation for Epigenetic Research Daniel Rose Dec 02, 2025 292 This