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This bias can distort apparent protein distributions, as evidenced by PolII mapping where sonicated chromatin showed a broad peak centered at the transcription start site, while MNase-based mapping accurately aligned with the polymerase's active site at +35 bp [49]
from the top result below
https://www.bioinforesearch.com/posts/histone-chip-crosslinking-methods-compared-from-formaldehyde-to-dual-fixation-for-epigenetic-research
In DNA, deoxyguanosine represents the primary target for formaldehyde crosslinking, with the Lys-dG coupling forming the predominant aminal linkage [6]
https://www.bioinforesearch.com/posts/overcoming-low-coverage-in-histone-chipseq-a-comprehensive-guide-from-foundational-concepts-to-clinical-application
How can I identify low coverage in my dataset